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synapsin i  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology synapsin i
    Synapsin I, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/synapsin+i/Synapsin+Ia%2Fb+Antibody/pmc12730897-78-131-133
    Average 93 stars, based on 69 article reviews
    synapsin i - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Wnt-5a Modulates Recycling of Functional GABA A Receptors on Hippocampal Neurons
    Article Snippet: Fixed cells were washed and incubated with an antibody to α1 or γ2 GABA A -Rs subunit (Santa Cruz Biotechnology Inc. and Sigma, respectively). .. For the staining of other proteins, cells were fixed as described above and permeabilized by incubation in PBS-0.2% Triton X-100, and stained with the following antibodies: synapsin I (Santa Cruz Biotechnology Inc.) and PSD-95 (UC Davis/NINDS/NIMH). .. Finally, cells were incubated with Alexa 543, Alexa 488, and/or Alexa 633 (Pierce) for 30 min at 37°C.

    Article Title: Wnt-5a Modulates Recycling of Functional GABAA Receptors on Hippocampal Neurons
    Article Snippet: Fixed cells were washed and incubated with an antibody to 1 or 2 GABAA-Rs subunit (Santa Cruz Biotechnology Inc. and Sigma, respectively). .. For the staining of other proteins, cells were fixed as described above and permeabilized by incubation in PBS-0.2% Triton X-100, and stained with the following antibodies: synapsin I (Santa Cruz Biotechnology Inc.) and PSD-95 (UC Davis/NINDS/NIMH). .. Finally, cells were incubated with Alexa 543, Alexa 488, and/or Alexa 633 (Pierce) for 30 min at 37°C.

    Incubation:

    Article Title: Wnt-5a Modulates Recycling of Functional GABA A Receptors on Hippocampal Neurons
    Article Snippet: Fixed cells were washed and incubated with an antibody to α1 or γ2 GABA A -Rs subunit (Santa Cruz Biotechnology Inc. and Sigma, respectively). .. For the staining of other proteins, cells were fixed as described above and permeabilized by incubation in PBS-0.2% Triton X-100, and stained with the following antibodies: synapsin I (Santa Cruz Biotechnology Inc.) and PSD-95 (UC Davis/NINDS/NIMH). .. Finally, cells were incubated with Alexa 543, Alexa 488, and/or Alexa 633 (Pierce) for 30 min at 37°C.

    Article Title: Enduring Effects of Humanin on Mitochondrial Systems in TBI Pathology
    Article Snippet: .. After blocking with 5% BSA, the membrane was incubated with primary antibody, p-Akt (Cell Signaling Technology, Danvers, MA, USA, Cat#4058S), Akt (Cell Signaling Technology, Danvers, MA, USA, Cat#9272S), p-AMPK (Cell Signaling Technology, Danvers, MA, USA, Cat#2535S), AMPK (Cell Signaling Technology, Danvers, MA, USA, Cat#2532S), LKB1 (Cell Signaling Technology, Danvers, MA, USA, Cat#3047S), PSD95 (Cell Signaling Technology, Danvers, MA, USA), p-STAT3 (Tyr705) (Cell Signaling Technology, Danvers, MA, USA, Cat#4113S), STAT3 (Cell Signaling Technology, Danvers, MA, USA, Cat#9139S), OXPHOS cocktail (Thermo Fisher Scientific, Waltham, MA, USA, Cat#45-8099), MFN2 (Cell Signaling Technology, Danvers, MA, USA, Cat#9482S), DRP1 (Cell Signaling Technology, Danvers, MA, USA, Cat#8570S), OPA1 (Cell Signaling Technology, Denvers, MA, USA, Cat#80471S), LC3B (Cell Signaling Technology, Denvers, MA, USA, Cat#4108S), PINK1 (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-517,353), parkin (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-32,282), synapsin I (Santa Cruz Biotechnology, Dallas, TX, USA, Catsc-376,623), GFAP (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-33,673), SOD2 (Cell Signaling Technology, Denvers, MA, USA, Cat#13141S, 1:2000), PGC-1α (Invitrogen, Waltham, MA, USA, Cat#PA5–72948, 1:2000), CC3 (Cell Signaling Technology, Denvers, MA, USA, Cat#9664, 1:600), and actin (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-47778) overnight at 4 °C. ..

    Article Title: Sex differences in the modulation of anxiety- and depression-like behaviors by matrix metalloproteinase-9 expression levels in mice
    Article Snippet: .. The following primary antibodies were incubated with the corresponding blocking solution at 4 °C overnight: BDNF (1:500, ab108319, Abcam, Cambridge, MA, USA), mTOR (1:1000, #4517, Cell Signaling, Massachusetts, USA), phospho-Ser2448-mTOR (1:1000, #2971, Cell Signaling, Massachusetts, USA), PSD95 (1:200, sc-8575, Santa Cruz Biotechnology, Texas, USA), synapsin I (1:200, sc-390867, Santa Cruz Biotechnology, Texas, USA), and β-tubulin III (1:20000, T2200/T8660, Sigma‒Aldrich, Missouri, USA). ..

    Article Title: Sex differences in the modulation of anxiety- and depression-like behaviors by matrix metalloproteinase-9 expression levels in mice.
    Article Snippet: .. The following primary antibodies were incubated with the corresponding blocking solution at 4 °C overnight: BDNF (1:500, ab108319, Abcam, Cambridge, MA, USA), mTOR (1:1000, #4517, Cell Signaling, Massachusetts, USA), phospho-Ser2448-mTOR (1:1000, #2971, Cell Signaling, Massachusetts, USA), PSD95 (1:200, sc-8575, Santa Cruz Biotechnology, Texas, USA), synapsin I (1:200, sc-390867, Santa Cruz Biotechnology, Texas, USA), and β-tubulin III (1:20000, T2200/T8660, Sigma‒Aldrich, Missouri, USA). ..

    Article Title: Wnt-5a Modulates Recycling of Functional GABAA Receptors on Hippocampal Neurons
    Article Snippet: Fixed cells were washed and incubated with an antibody to 1 or 2 GABAA-Rs subunit (Santa Cruz Biotechnology Inc. and Sigma, respectively). .. For the staining of other proteins, cells were fixed as described above and permeabilized by incubation in PBS-0.2% Triton X-100, and stained with the following antibodies: synapsin I (Santa Cruz Biotechnology Inc.) and PSD-95 (UC Davis/NINDS/NIMH). .. Finally, cells were incubated with Alexa 543, Alexa 488, and/or Alexa 633 (Pierce) for 30 min at 37°C.

    Blocking Assay:

    Article Title: Enduring Effects of Humanin on Mitochondrial Systems in TBI Pathology
    Article Snippet: .. After blocking with 5% BSA, the membrane was incubated with primary antibody, p-Akt (Cell Signaling Technology, Danvers, MA, USA, Cat#4058S), Akt (Cell Signaling Technology, Danvers, MA, USA, Cat#9272S), p-AMPK (Cell Signaling Technology, Danvers, MA, USA, Cat#2535S), AMPK (Cell Signaling Technology, Danvers, MA, USA, Cat#2532S), LKB1 (Cell Signaling Technology, Danvers, MA, USA, Cat#3047S), PSD95 (Cell Signaling Technology, Danvers, MA, USA), p-STAT3 (Tyr705) (Cell Signaling Technology, Danvers, MA, USA, Cat#4113S), STAT3 (Cell Signaling Technology, Danvers, MA, USA, Cat#9139S), OXPHOS cocktail (Thermo Fisher Scientific, Waltham, MA, USA, Cat#45-8099), MFN2 (Cell Signaling Technology, Danvers, MA, USA, Cat#9482S), DRP1 (Cell Signaling Technology, Danvers, MA, USA, Cat#8570S), OPA1 (Cell Signaling Technology, Denvers, MA, USA, Cat#80471S), LC3B (Cell Signaling Technology, Denvers, MA, USA, Cat#4108S), PINK1 (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-517,353), parkin (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-32,282), synapsin I (Santa Cruz Biotechnology, Dallas, TX, USA, Catsc-376,623), GFAP (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-33,673), SOD2 (Cell Signaling Technology, Denvers, MA, USA, Cat#13141S, 1:2000), PGC-1α (Invitrogen, Waltham, MA, USA, Cat#PA5–72948, 1:2000), CC3 (Cell Signaling Technology, Denvers, MA, USA, Cat#9664, 1:600), and actin (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-47778) overnight at 4 °C. ..

    Article Title: Sex differences in the modulation of anxiety- and depression-like behaviors by matrix metalloproteinase-9 expression levels in mice
    Article Snippet: .. The following primary antibodies were incubated with the corresponding blocking solution at 4 °C overnight: BDNF (1:500, ab108319, Abcam, Cambridge, MA, USA), mTOR (1:1000, #4517, Cell Signaling, Massachusetts, USA), phospho-Ser2448-mTOR (1:1000, #2971, Cell Signaling, Massachusetts, USA), PSD95 (1:200, sc-8575, Santa Cruz Biotechnology, Texas, USA), synapsin I (1:200, sc-390867, Santa Cruz Biotechnology, Texas, USA), and β-tubulin III (1:20000, T2200/T8660, Sigma‒Aldrich, Missouri, USA). ..

    Article Title: Sex differences in the modulation of anxiety- and depression-like behaviors by matrix metalloproteinase-9 expression levels in mice.
    Article Snippet: .. The following primary antibodies were incubated with the corresponding blocking solution at 4 °C overnight: BDNF (1:500, ab108319, Abcam, Cambridge, MA, USA), mTOR (1:1000, #4517, Cell Signaling, Massachusetts, USA), phospho-Ser2448-mTOR (1:1000, #2971, Cell Signaling, Massachusetts, USA), PSD95 (1:200, sc-8575, Santa Cruz Biotechnology, Texas, USA), synapsin I (1:200, sc-390867, Santa Cruz Biotechnology, Texas, USA), and β-tubulin III (1:20000, T2200/T8660, Sigma‒Aldrich, Missouri, USA). ..

    Membrane:

    Article Title: Enduring Effects of Humanin on Mitochondrial Systems in TBI Pathology
    Article Snippet: .. After blocking with 5% BSA, the membrane was incubated with primary antibody, p-Akt (Cell Signaling Technology, Danvers, MA, USA, Cat#4058S), Akt (Cell Signaling Technology, Danvers, MA, USA, Cat#9272S), p-AMPK (Cell Signaling Technology, Danvers, MA, USA, Cat#2535S), AMPK (Cell Signaling Technology, Danvers, MA, USA, Cat#2532S), LKB1 (Cell Signaling Technology, Danvers, MA, USA, Cat#3047S), PSD95 (Cell Signaling Technology, Danvers, MA, USA), p-STAT3 (Tyr705) (Cell Signaling Technology, Danvers, MA, USA, Cat#4113S), STAT3 (Cell Signaling Technology, Danvers, MA, USA, Cat#9139S), OXPHOS cocktail (Thermo Fisher Scientific, Waltham, MA, USA, Cat#45-8099), MFN2 (Cell Signaling Technology, Danvers, MA, USA, Cat#9482S), DRP1 (Cell Signaling Technology, Danvers, MA, USA, Cat#8570S), OPA1 (Cell Signaling Technology, Denvers, MA, USA, Cat#80471S), LC3B (Cell Signaling Technology, Denvers, MA, USA, Cat#4108S), PINK1 (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-517,353), parkin (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-32,282), synapsin I (Santa Cruz Biotechnology, Dallas, TX, USA, Catsc-376,623), GFAP (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-33,673), SOD2 (Cell Signaling Technology, Denvers, MA, USA, Cat#13141S, 1:2000), PGC-1α (Invitrogen, Waltham, MA, USA, Cat#PA5–72948, 1:2000), CC3 (Cell Signaling Technology, Denvers, MA, USA, Cat#9664, 1:600), and actin (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-47778) overnight at 4 °C. ..

    Pyrolysis Gas Chromatography:

    Article Title: Enduring Effects of Humanin on Mitochondrial Systems in TBI Pathology
    Article Snippet: .. After blocking with 5% BSA, the membrane was incubated with primary antibody, p-Akt (Cell Signaling Technology, Danvers, MA, USA, Cat#4058S), Akt (Cell Signaling Technology, Danvers, MA, USA, Cat#9272S), p-AMPK (Cell Signaling Technology, Danvers, MA, USA, Cat#2535S), AMPK (Cell Signaling Technology, Danvers, MA, USA, Cat#2532S), LKB1 (Cell Signaling Technology, Danvers, MA, USA, Cat#3047S), PSD95 (Cell Signaling Technology, Danvers, MA, USA), p-STAT3 (Tyr705) (Cell Signaling Technology, Danvers, MA, USA, Cat#4113S), STAT3 (Cell Signaling Technology, Danvers, MA, USA, Cat#9139S), OXPHOS cocktail (Thermo Fisher Scientific, Waltham, MA, USA, Cat#45-8099), MFN2 (Cell Signaling Technology, Danvers, MA, USA, Cat#9482S), DRP1 (Cell Signaling Technology, Danvers, MA, USA, Cat#8570S), OPA1 (Cell Signaling Technology, Denvers, MA, USA, Cat#80471S), LC3B (Cell Signaling Technology, Denvers, MA, USA, Cat#4108S), PINK1 (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-517,353), parkin (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-32,282), synapsin I (Santa Cruz Biotechnology, Dallas, TX, USA, Catsc-376,623), GFAP (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-33,673), SOD2 (Cell Signaling Technology, Denvers, MA, USA, Cat#13141S, 1:2000), PGC-1α (Invitrogen, Waltham, MA, USA, Cat#PA5–72948, 1:2000), CC3 (Cell Signaling Technology, Denvers, MA, USA, Cat#9664, 1:600), and actin (Santacruz Biotechnology, Dallas, TX, USA, Cat#sc-47778) overnight at 4 °C. ..



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    NRMS upregulates transcriptional levels of genes related to synaptic plasticity in damaged region of SCI rats. (A) The functional classification of different genes in the SCI + NRMS and SCI + SS groups by GO enrichment analysis. (B) The functional classification of different genes between the SCI + NRMS and SCI + SS groups by KEGG database. (C–F) The relative levels of Syn2 , Syp , GAP43 , and MAP2 mRNA normalized by the Sham group. The bold lines, upper boundaries, and lower boundaries represent the medians, 75 th percentiles, and 25 th percentiles, respectively. Whiskers extend 1.5 times interquartile range ( n = 4 per group). * P < 0.05, vs . Sham group; # P < 0.05, vs . SCI + SS group. GAP43: Growth-associated protein 43; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MAP2: microtubule-associated protein 2; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation; Syn2: <t>synapsin</t> <t>II;</t> Syp: synaptophysin.
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    NRMS upregulates transcriptional levels of genes related to synaptic plasticity in damaged region of SCI rats. (A) The functional classification of different genes in the SCI + NRMS and SCI + SS groups by GO enrichment analysis. (B) The functional classification of different genes between the SCI + NRMS and SCI + SS groups by KEGG database. (C–F) The relative levels of Syn2 , Syp <t>,</t> <t>GAP43</t> , and MAP2 mRNA normalized by the Sham group. The bold lines, upper boundaries, and lower boundaries represent the medians, 75 th percentiles, and 25 th percentiles, respectively. Whiskers extend 1.5 times interquartile range ( n = 4 per group). * P < 0.05, vs . Sham group; # P < 0.05, vs . SCI + SS group. GAP43: Growth-associated protein 43; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MAP2: microtubule-associated protein 2; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation; Syn2: <t>synapsin</t> II; Syp: synaptophysin.
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    Image Search Results


    NRMS upregulates transcriptional levels of genes related to synaptic plasticity in damaged region of SCI rats. (A) The functional classification of different genes in the SCI + NRMS and SCI + SS groups by GO enrichment analysis. (B) The functional classification of different genes between the SCI + NRMS and SCI + SS groups by KEGG database. (C–F) The relative levels of Syn2 , Syp , GAP43 , and MAP2 mRNA normalized by the Sham group. The bold lines, upper boundaries, and lower boundaries represent the medians, 75 th percentiles, and 25 th percentiles, respectively. Whiskers extend 1.5 times interquartile range ( n = 4 per group). * P < 0.05, vs . Sham group; # P < 0.05, vs . SCI + SS group. GAP43: Growth-associated protein 43; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MAP2: microtubule-associated protein 2; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation; Syn2: synapsin II; Syp: synaptophysin.

    Journal: Neural Regeneration Research

    Article Title: Nerve root magnetic stimulation regulates the synaptic plasticity of injured spinal cord by ascending sensory pathway

    doi: 10.4103/NRR.NRR-D-24-00628

    Figure Lengend Snippet: NRMS upregulates transcriptional levels of genes related to synaptic plasticity in damaged region of SCI rats. (A) The functional classification of different genes in the SCI + NRMS and SCI + SS groups by GO enrichment analysis. (B) The functional classification of different genes between the SCI + NRMS and SCI + SS groups by KEGG database. (C–F) The relative levels of Syn2 , Syp , GAP43 , and MAP2 mRNA normalized by the Sham group. The bold lines, upper boundaries, and lower boundaries represent the medians, 75 th percentiles, and 25 th percentiles, respectively. Whiskers extend 1.5 times interquartile range ( n = 4 per group). * P < 0.05, vs . Sham group; # P < 0.05, vs . SCI + SS group. GAP43: Growth-associated protein 43; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MAP2: microtubule-associated protein 2; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation; Syn2: synapsin II; Syp: synaptophysin.

    Article Snippet: In addition, Synapsin I and Synapsin II exert essential roles in neurotransmitter release (Cesca et al., 2010).

    Techniques: Functional Assay

    NRMS alters expression of proteins associated with synaptic plasticity in the injured spinal cord of rats. (A) Representative western blots show the expression levels of PSD95, GAP43, Synapsin I and Synapsin II collected from the damaged area of spinal cord on day 22 after SCI. (B–E) The semi-quantified results of PSD95, GAP43, Synapsin I, and Synapsin II protein levels. All experiments were repeated three times. Data are expressed as mean ± SD ( n = 5 per group). ** P < 0.01, *** P < 0.001, vs. Sham group; # P < 0.05, ## P < 0.01, vs . SCI + SS group. GAP43: Growth-associated protein 43; PSD95: postsynaptic density protein-95; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation.

    Journal: Neural Regeneration Research

    Article Title: Nerve root magnetic stimulation regulates the synaptic plasticity of injured spinal cord by ascending sensory pathway

    doi: 10.4103/NRR.NRR-D-24-00628

    Figure Lengend Snippet: NRMS alters expression of proteins associated with synaptic plasticity in the injured spinal cord of rats. (A) Representative western blots show the expression levels of PSD95, GAP43, Synapsin I and Synapsin II collected from the damaged area of spinal cord on day 22 after SCI. (B–E) The semi-quantified results of PSD95, GAP43, Synapsin I, and Synapsin II protein levels. All experiments were repeated three times. Data are expressed as mean ± SD ( n = 5 per group). ** P < 0.01, *** P < 0.001, vs. Sham group; # P < 0.05, ## P < 0.01, vs . SCI + SS group. GAP43: Growth-associated protein 43; PSD95: postsynaptic density protein-95; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation.

    Article Snippet: In addition, Synapsin I and Synapsin II exert essential roles in neurotransmitter release (Cesca et al., 2010).

    Techniques: Expressing, Western Blot

    NRMS upregulates transcriptional levels of genes related to synaptic plasticity in damaged region of SCI rats. (A) The functional classification of different genes in the SCI + NRMS and SCI + SS groups by GO enrichment analysis. (B) The functional classification of different genes between the SCI + NRMS and SCI + SS groups by KEGG database. (C–F) The relative levels of Syn2 , Syp , GAP43 , and MAP2 mRNA normalized by the Sham group. The bold lines, upper boundaries, and lower boundaries represent the medians, 75 th percentiles, and 25 th percentiles, respectively. Whiskers extend 1.5 times interquartile range ( n = 4 per group). * P < 0.05, vs . Sham group; # P < 0.05, vs . SCI + SS group. GAP43: Growth-associated protein 43; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MAP2: microtubule-associated protein 2; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation; Syn2: synapsin II; Syp: synaptophysin.

    Journal: Neural Regeneration Research

    Article Title: Nerve root magnetic stimulation regulates the synaptic plasticity of injured spinal cord by ascending sensory pathway

    doi: 10.4103/NRR.NRR-D-24-00628

    Figure Lengend Snippet: NRMS upregulates transcriptional levels of genes related to synaptic plasticity in damaged region of SCI rats. (A) The functional classification of different genes in the SCI + NRMS and SCI + SS groups by GO enrichment analysis. (B) The functional classification of different genes between the SCI + NRMS and SCI + SS groups by KEGG database. (C–F) The relative levels of Syn2 , Syp , GAP43 , and MAP2 mRNA normalized by the Sham group. The bold lines, upper boundaries, and lower boundaries represent the medians, 75 th percentiles, and 25 th percentiles, respectively. Whiskers extend 1.5 times interquartile range ( n = 4 per group). * P < 0.05, vs . Sham group; # P < 0.05, vs . SCI + SS group. GAP43: Growth-associated protein 43; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MAP2: microtubule-associated protein 2; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation; Syn2: synapsin II; Syp: synaptophysin.

    Article Snippet: The following antibodies were used: postsynaptic density protein-95 (PSD95; rabbit, 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 3450S, RRID: AB_2292883), Synapsin I (rabbit, 1:1000, Abmart, Shanghai, China, Cat# 334286), GAP43 (mouse, 1:400, Santa Cruz, Biotechnology, Dallas, TX, USA, Cat# sc-17790, RRID: AB_627660), Synapsin II (rabbit, 1:1000, Abclonal, Wuhan, China, Cat# A19542), and GAPDH (mouse, 1:20 000, Sigma-Aldrich, Cat# G8795, RRID: AB_1078991).

    Techniques: Functional Assay

    NRMS alters expression of proteins associated with synaptic plasticity in the injured spinal cord of rats. (A) Representative western blots show the expression levels of PSD95, GAP43, Synapsin I and Synapsin II collected from the damaged area of spinal cord on day 22 after SCI. (B–E) The semi-quantified results of PSD95, GAP43, Synapsin I, and Synapsin II protein levels. All experiments were repeated three times. Data are expressed as mean ± SD ( n = 5 per group). ** P < 0.01, *** P < 0.001, vs. Sham group; # P < 0.05, ## P < 0.01, vs . SCI + SS group. GAP43: Growth-associated protein 43; PSD95: postsynaptic density protein-95; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation.

    Journal: Neural Regeneration Research

    Article Title: Nerve root magnetic stimulation regulates the synaptic plasticity of injured spinal cord by ascending sensory pathway

    doi: 10.4103/NRR.NRR-D-24-00628

    Figure Lengend Snippet: NRMS alters expression of proteins associated with synaptic plasticity in the injured spinal cord of rats. (A) Representative western blots show the expression levels of PSD95, GAP43, Synapsin I and Synapsin II collected from the damaged area of spinal cord on day 22 after SCI. (B–E) The semi-quantified results of PSD95, GAP43, Synapsin I, and Synapsin II protein levels. All experiments were repeated three times. Data are expressed as mean ± SD ( n = 5 per group). ** P < 0.01, *** P < 0.001, vs. Sham group; # P < 0.05, ## P < 0.01, vs . SCI + SS group. GAP43: Growth-associated protein 43; PSD95: postsynaptic density protein-95; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation.

    Article Snippet: The following antibodies were used: postsynaptic density protein-95 (PSD95; rabbit, 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 3450S, RRID: AB_2292883), Synapsin I (rabbit, 1:1000, Abmart, Shanghai, China, Cat# 334286), GAP43 (mouse, 1:400, Santa Cruz, Biotechnology, Dallas, TX, USA, Cat# sc-17790, RRID: AB_627660), Synapsin II (rabbit, 1:1000, Abclonal, Wuhan, China, Cat# A19542), and GAPDH (mouse, 1:20 000, Sigma-Aldrich, Cat# G8795, RRID: AB_1078991).

    Techniques: Expressing, Western Blot